| ZHU Wei-yao,LIU Rui,ZHAN De-song,REN Ling,WANG Qiang.Biocompatibility between Cu-bearing CoCrW Alloy by 3D Printing and PDLF[J],48(7):324-331 |
| Biocompatibility between Cu-bearing CoCrW Alloy by 3D Printing and PDLF |
| Received:October 25, 2018 Revised:July 20, 2019 |
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| DOI：10.16490/j.cnki.issn.1001-3660.2019.07.037 |
| KeyWord:CoCrW alloy Cu selected laser melting (SLM) biocompatibility cytotoxicity |
| Author | Institution |
| ZHU Wei-yao |
1.China Medical University, Shenyang , China |
| LIU Rui |
2.Institute of Metal Research, Chinese Academy of Sciences, Shenyang , China |
| ZHAN De-song |
1.China Medical University, Shenyang , China |
| REN Ling |
2.Institute of Metal Research, Chinese Academy of Sciences, Shenyang , China |
| WANG Qiang |
1.China Medical University, Shenyang , China |
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| Abstract: |
| The work aims to add Cu in existing medical CoCrW alloy to prepare a new Cu-bearing CoCrW alloy by selected laser melting (SLM) and test the biocompatibility. The new Cu-bearing CoCrW alloy prepared by SLM was used as the experi-mental group and the Cu-free CoCrW alloy prepared by SLM was regarded as the control group. After the experimental materials and periodontalliga-ment fibroblast were co-cultured, CCK-8 and Real Time Cellular Analysis (RTCA, cytotoxicity assay, Phalloidin cell staining and flow cytometry) were adopted to test the effects of materials on proliferation, apoptosis and adhesion of cells so as to evaluate the biocompatibility of new Cu-bearing CoCrW alloy. The RGR of PDLF cells directly contacting the new Cu-bearing CoCrW alloys after co-cultured was more than 99% and the cells were classified as zero-grade cytotoxicity. The growth of PDLF cells indirectly contacting the new Cu-bearing CoCrW alloys after co-cultured was not affected by the new alloy. The apoptosis rate of PDLF was less than 1% after co-cultured with CoCrW-Cu alloy for 1 day and less than 5% after 3 days of co-culture. There was no significant difference between the experimental group and the control group and the apoptosis rate of experimental group was lower than that of control group. Through the Phalloidin cell staining, the cell adhesion was normal and there was no significant difference between the experimental group and the control group. The Cu-bearing CoCrW-Cu alloy fabricated by SLM technology has good antibacterial performance and no cytotoxicity to cells, that is, good cell compatibility. |
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