刘国梁,李程程,张凤,邢宏宇,孙树峰,冀敏.水热及化学处理对烧结羟基磷灰石生物活性的影响[J].表面技术,2025,54(10):225-236.
LIU Guoliang,LI Chengcheng,ZHANG Feng,XING Hongyu,SUN Shufeng,JI Min.Effect of Hydrothermal and Chemical Treatment on Bioactivity of Sintered Hydroxyapatite[J].Surface Technology,2025,54(10):225-236 |
| 水热及化学处理对烧结羟基磷灰石生物活性的影响 |
| Effect of Hydrothermal and Chemical Treatment on Bioactivity of Sintered Hydroxyapatite |
| 投稿时间：2024-08-04 修订日期：2025-01-06 |
| DOI：10.16490/j.cnki.issn.1001-3660.2025.10.018 |
| 中文关键词: 烧结羟基磷灰石 细胞增殖 水热处理 氢氧化钠处理 氟化钠处理 润湿性 |
| 英文关键词:sintered hydroxyapatite cell proliferation hydrothermal NaOH treatment NaF soaking wettability |
| 基金项目:国家自然科学基金资助项目(52375446，52305478);山东省自然科学基金资助项目(ZR2023QE178) |
| 作者 | 单位 |
| 刘国梁 | 青岛理工大学 机械与汽车工程学院，山东 青岛 266520 |
| 李程程 | 青岛理工大学 机械与汽车工程学院，山东 青岛 266520 |
| 张凤 | 山东大学 生命科学学院，山东 青岛 266237 |
| 邢宏宇 | 山东建筑大学 机电工程学院，济南 250101 |
| 孙树峰 | 青岛理工大学 机械与汽车工程学院，山东 青岛 266520 |
| 冀敏 | 青岛理工大学 机械与汽车工程学院，山东 青岛 266520 |
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| Author | Institution |
| LIU Guoliang | School of Mechanical & Automotive Engineering, Qingdao University of Technology, Shandong Qingdao 266520, China |
| LI Chengcheng | School of Mechanical & Automotive Engineering, Qingdao University of Technology, Shandong Qingdao 266520, China |
| ZHANG Feng | School of Life Sciences, Shandong University, Shandong Qingdao 266237, China |
| XING Hongyu | School of Mechanical and Electrical Engineering, Shandong Jianzhu University, Jinan 250101, China |
| SUN Shufeng | School of Mechanical & Automotive Engineering, Qingdao University of Technology, Shandong Qingdao 266520, China |
| JI Min | School of Mechanical & Automotive Engineering, Qingdao University of Technology, Shandong Qingdao 266520, China |
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| 中文摘要: |
| 目的 烧结羟基磷灰石(Sintered Hydroxyapatite，S-HAp)在骨修复领域具有极高的应用潜力，但其细胞生物活性不足，必须探索改善S-HAp细胞生物活性的方法。方法 研究了水热、氢氧化钠蒸煮和氟化钠浸泡3种表面处理方法以及水热温度、氢氧化钠/氟化钠浓度等工艺参数对S-HAp表面成骨细胞增殖活性的影响规律，并基于处理前后S-HAp的表面形貌、元素组成、润湿性、pH值等揭示了各表面处理方法对成骨性能的影响机制。结果 水热处理可在S-HAp表面腐蚀产生大量多孔结构进而增强其亲水特性，从而改善细胞增殖活性，并且水热温度越高对细胞增殖活性的改善效果越明显;氢氧化钠处理则可在S-HAp表面沉积NaOH晶体，从而中和细胞生长和增殖过程中产生的酸性物质，使细胞培养基维持更长时间的弱碱性环境以提高细胞增殖活性，但NaOH浓度过高会导致培养基过碱性，抑制细胞的初期生长和增殖;氟化钠可与S-HAp发生化学反应，在S-HAp表面引入氟离子以刺激细胞生长和增殖，实现细胞增殖活性的改善，但氟离子浓度过高会对细胞产生毒害。结论 3种处理方法均可以提升烧结羟基磷灰石的细胞增殖活性，但氢氧化钠结晶调节pH的效果会受到植入物周边组织液含量和流速的影响，可控性差，水热和氟化钠处理更适合作为提高烧结羟基磷灰石植入物细胞增殖活性的方法。 |
| 英文摘要: |
| Sintered hydroxyapatite (S-HAp) has great potential for applications in bone repair, but its insufficient cellular bioactivity limits the use. To improve the cellular bioactivity of S-HAp, this study investigated the effects of three surface treatment methods- hydrothermal, sodium hydroxide (NaOH) boiling, and sodium fluoride (NaF) soaking-along with various process parameters such as hydrothermal temperature and NaOH/NaF concentration on the proliferation activity of osteoblasts on S-HAp surfaces. The mechanisms were revealed based on the surface morphology, elemental composition, wettability, pH, and other characteristics of S-HAp before and after treatment. The results indicated that hydrothermal treatment could create a large number of loose and porous structures on the S-HAp surface, enhancing its hydrophilicity and thus improving cellular proliferation activity; moreover, higher hydrothermal temperatures led to great pronounced improvement in cellular proliferation. The 1, 3 and 7 d values of OD 450 of S-HAp treated by hydrothermal at 240 ℃ were almost 50% higher than that of the control group. NaOH treatment could deposit NaOH crystals on the S-HAp surface, neutralizing the acidic substances produced during cell growth and proliferation, allowing the cell culture medium to maintain a weakly alkaline environment for a longer time and thereby enhancing cellular proliferation activity. The 0.4 mol /l NaOH treatment improved the 7 d OD 450 value of S-HAp by 75% compared with the control group. However, excessively high NaOH concentration could lead to an overly alkaline medium, inhibiting initial cell growth and proliferation. The 1 d OD 450 values of S-HAp treated by 0.8 and 1.6 mol/L NaOH were both lower than that of the control group. As time went on, the acidic substances produced during cell growth and proliferation continually accumulated, leading the pH to a suitable range, and then the OD 450 values of S-HAp treated by 0.8 and 1.6 mol/L NaOH exceeded that of the control group. Additionally, NaF could chemically react with S-HAp, introducing fluoride ions on the S-HAp surface to stimulate cell growth and proliferation, improving cellular proliferation activity. However, excessive fluoride ion concentrations could be toxic to the cells. During the experimental range, the S-HAp samples soaked by NaF at various concentrations all obtained larger OD 450 values than the control group, but the values continuously decreased as the NaF concentration increased. The three treatment methods, hydrothermal, NaOH boiling, and NaF soaking, all can enhance the cell proliferation activity of sintered hydroxyapatite. By comparing the effects of different treatments on the proliferation activity of S-HAp samples, it can be seen that the 0.4 mol/L NaOH treatment obtained the best improvement effect in the experimental range. However, the pH regulation function of sodium hydroxide can be affected by the content and flow rate of tissue fluid around the implant, and the effect is poorly controllable. Therefore, hydrothermal and sodium fluoride treatment are more suitable for improving the cell proliferation activity of sintered hydroxyapatite implants. But it should be noted that the effect of hydrothermal treatment on the strength and wear-resisting property of implants should be further studied, while the poisonousness of implants with sodium fluoride treatment must be tightly verified one by one before clinical application. |
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