曹慧烨,陈着,张赫,柏广行,朱叶,李小杰.尿路导管表面含胍基聚合物生物涂层的制备及性能研究[J].表面技术,2025,54(2):222-233.
CAO Huiye,CHEN Zhuo,ZHANG He,BAI Guanghang,ZHU Ye,LI Xiaojie.Preparation and Antibacterial Properties of Guanidine-based Polymer Coatings on the Surface of Urinary Catheters[J].Surface Technology,2025,54(2):222-233 |
| 尿路导管表面含胍基聚合物生物涂层的制备及性能研究 |
| Preparation and Antibacterial Properties of Guanidine-based Polymer Coatings on the Surface of Urinary Catheters |
| 投稿时间：2024-01-26 修订日期：2024-03-27 |
| DOI：10.16490/j.cnki.issn.1001-3660.2025.02.019 |
| 中文关键词: 胍 导尿管 涂层 抗菌 低毒性 光固化 |
| 英文关键词:guanidine urinary catheters coatings antimicrobial biocompatibility UV curing |
| 基金项目:国家自然科学基金面上项目(52173127) |
| 作者 | 单位 |
| 曹慧烨 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
| 陈着 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
| 张赫 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
| 柏广行 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
| 朱叶 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
| 李小杰 | 江南大学 化学与材料工程学院，江苏 无锡 214122 |
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| Author | Institution |
| CAO Huiye | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
| CHEN Zhuo | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
| ZHANG He | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
| BAI Guanghang | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
| ZHU Ye | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
| LI Xiaojie | School of Chemical and Material Engineering, Jiangnan University, Jiangsu Wuxi 214122, China |
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| 中文摘要: |
| 目的 制备一种含胍基的聚合物抗菌涂层，实现其在尿路导管上的应用。方法 以甲基丙烯酸羟乙酯(HEMA)、N-(3-氨基丙基)甲基丙烯酰胺盐酸盐(APMA)和4-甲基丙烯酰氧基二苯甲酮(BP)作为共聚单体，通过自由基聚合制备了一种侧基带伯胺基的三元共聚物ploy(HEMA-co-APMA-co-BP), PHAB。随后通过胍基化改性将伯胺基团转变为胍基。利用浸涂法在聚氨酯表面制备含胍基聚合物抗菌涂层，随后进行紫外光固化使涂层进行交联固化，得到含胍基聚合物抗菌涂层(TPU-PHGB)。对涂层的基本性能进行了表征。通过涂板法定量评估涂层对大肠杆菌、金黄色葡萄球菌和尿路中促进结石产生的奇异变形杆菌的初始和长久抗菌性能。并测试了3种含胍基抗菌聚合物的最小抑菌浓度和最小杀菌浓度。后续在体外模拟膀胱模型中培养7 d，观察涂层表面结石的生长情况，评估其抗结垢性能。通过活死细胞染色实验，并利用CCK-8试剂盒测试涂层及聚合物的细胞毒性，评估涂层的生物相容性。结果 涂层表面均一光滑，具有长久的广谱抗菌性和良好的生物相容性，并且可以减少表面结石的产生。在与大肠杆菌、金黄色葡萄球菌和奇异变形杆菌共培养10 h后，涂层的抗菌效率可达99%;在共培养24 h和48 h后，涂层对大肠杆菌的抗菌效率在80%以上，对金黄色葡萄球菌和奇异变形杆菌的抗菌效率都保持在95%以上;在长达168 h共培养后，涂层对大肠杆菌的抗菌效率仍有65%以上，对金黄色葡萄球菌的抗菌效率为85%;对奇异变形杆菌的抗菌效率高达90%以上。在体外模拟膀胱模型中，涂层有效减少了结石的形成。结论 涂层不仅具有初始和长久广谱抗菌性，而且在体外抗结垢模型中减少了表面结石的产生，并且具有良好的生物相容性。 |
| 英文摘要: |
| During the catheter implantation process, the adhesion and colonization of bacteria, along with the attachment of extracellular polymeric substances (EPS), have the potential to result in hospital-acquired infections (HAI) and the development of surface biofilms. Moreover, the urease produced by proteus mirabilis enzymatically degrades urea, leading to an alkaline environment on the catheter surface and consequently promoting the increased formation of stones, including calcium phosphate and magnesium phosphate. As a result, achieving effective and enduring antimicrobial properties remains one of the most formidable challenges in the field of catheters. The guanidine exhibiting more efficient antimicrobial properties compared to quaternary ammonium salt and lower cytotoxicity compared to amine was utilized to prepare an antimicrobial coating on the polymer surface. By employing hydroxyethyl methacrylate (HEMA), N-(3-aminopropyl) methacrylamide hydrochloride (APMA), and 4-methacryloyloxybenzophenone (BP) as monomers, ploy(HEMA-co-APMA-co-BP) and PHAB with amine-functionalized side chains were prepared via free radical polymerization. Subsequently, the amine groups on PHAB were modified into guanidine groups (PHGB) by guanidino modification. Thus, the application of guanidine-based polymers in antimicrobial surface coatings for urinary catheters was facilitated. |
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