周楠,李梦茹,黄楠,汪川哲,王文轩,涂秋芬,熊开琴.具有抗炎促内皮细胞生长的多功能药物洗脱支架的制备及表征[J].表面技术,2024,53(24):216-227.
ZHOU Nan,LI Mengru,HUANG Nan,WANG Chuanzhe,WANG Wenxuan,TU Qiufen,XIONG Kaiqin.Preparation and Characterization of a Multifunctional Drug-eluting Stent with Anti-inflammatory and Endothelial Cell Growth-promoting[J].Surface Technology,2024,53(24):216-227 |
| 具有抗炎促内皮细胞生长的多功能药物洗脱支架的制备及表征 |
| Preparation and Characterization of a Multifunctional Drug-eluting Stent with Anti-inflammatory and Endothelial Cell Growth-promoting |
| 投稿时间：2024-01-17 修订日期：2024-05-30 |
| DOI：10.16490/j.cnki.issn.1001-3660.2024.24.020 |
| 中文关键词: 动脉粥样硬化 炎症 洗脱支架 丹酚酸B 超声雾化喷涂技术 |
| 英文关键词:atherosclerosis inflammation drug eluting stent salvianolic acid B ultrasonic atomization spraying |
| 基金项目: |
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| Author | Institution |
| ZHOU Nan | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| LI Mengru | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| HUANG Nan | Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| WANG Chuanzhe | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| WANG Wenxuan | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| TU Qiufen | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
| XIONG Kaiqin | Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China;Key Laboratory of Advanced Technologies of Materials, Ministry of Education, College of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China |
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| 中文摘要: |
| 目的 血管支架植入是动脉粥样硬化心血管疾病最主要的临床治疗手段。丹酚酸B(Sal B)是从丹参中提取的生物活性化合物，被证实具有较强的抗炎功能及改善心血管功能的潜力，但目前的研究集中于通过静脉注射的给药方式干预心血管疾病，该方法存在药物生物利用度低等缺点。基于此，以聚乳酸-羟基乙酸共聚物(PLGA)为药物释放载体，在血管支架表面构建Sal B药物洗脱涂层，旨在实现药物在病灶处的局部释放并提高支架长期功能。方法 利用超声雾化喷涂技术在血管支架表面构建Sal B/PLGA涂层。通过傅里叶变换红外吸收光谱仪(FTIR)、紫外分光光度计(UV-Vis)和场发射扫描电子显微镜(SEM)等表征手段对涂层的化学结构、药物释放行为和表面形貌进行检测。体外细胞实验用于评价该涂层的抗炎能力以及对内皮细胞(ECs)、平滑肌细胞(SMCs)的增殖影响。最后通过体外血液相容性和半体内血液循环实验评价该涂层的血液相容性以及抗血栓性能。结果 材料学表征结果证明Sal B/PLGA涂层构建成功，且涂层力学性能良好，可实现Sal B长达28 d的有效释放。细胞生物学结果表明该涂层具有一定的抗炎功能，能在促进内皮细胞的同时抑制平滑肌细胞的异常增殖。血液相容性及半体内循环实验结果表明，该涂层能有效减少血小板的黏附、聚集与激活，具有抗血栓的能力。结论 Sal B/PLGA血管支架涂层表现出潜在的抗炎、促内皮、抑制平滑肌增殖及抗血栓等特性，可为后续提高支架治疗功效提供一种创新策略。 |
| 英文摘要: |
| The implantation of vascular stents is popularly adopted to treat atherosclerotic cardiovascular diseases. Salvianolic acid B (Sal B), a bioactive compound extracted from Salvia miltiorrhiza, has been gradually substantiated for its robust anti-inflammatory properties and its potential to enhance cardiovascular function. Nonetheless, the current state of research predominantly concentrates on addressing cardiovascular diseases through intravenous administration, a method fraught with limitations such as suboptimal drug bioavailability. Based on the current knowledge, the current study employs poly (lactic-co-glycolic acid) (PLGA) as a drug carrier to fabricate Sal B eluting coating (Sal B/PLGA) on the surface of vascular stents. It is designed to achieve localized drug release at the site of the lesion and improve the long-term function of the stent. The work aims to enhance the long-term functionality of the stents, providing an innovative strategy for improving the effectiveness of stent treatments in the future. The Sal B/PLGA coating was meticulously developed on the surface of vascular stents through the utilization of ultrasound-assisted spray coating technology and comprehensively characterized via multiple techniques. The chemical structure, drug release behavior and surface morphology of the coating were characterized via Fourier-transform infrared spectroscopy (FTIR), ultraviolet-visible spectrophotometry (UV-Vis), and field emission scanning electron microscopy (SEM), respectively. In vitro cellular experiments were conducted to evaluate the impact of the coating on the proliferation of endothelial cells (ECs) and smooth muscle cells (SMCs), as well as its anti-inflammatory ability. In the final phase of the research, the blood compatibility and anti-thrombotic properties of the coating were validated through in vitro blood compatibility assays. Furthermore, ex vivo blood circulation experiments were performed to provide a more realistic assessment of the coating performance in a physiological context. Material characterization results indicated the successful construction of the Sal B/PLGA coating on the vascular stent. The coating exhibited favorable mechanical properties, allowing it to withstand deformation caused by stent expansion, and ensured efficient release of Sal B for over 28 days. The results of cell biology indicated that the coating had anti-inflammatory effects. It not only promoted the functionality of endothelial cells, but also concurrently restrained the abnormal proliferation of smooth muscle cells. Furthermore, insights from blood compatibility and ex vivo circulation experiments indicated the notable ability of the coating to reduce platelet adhesion, aggregation, and activation. These findings affirm its anti-thrombotic functionality, effectively reducing the risk of post-stent implantation coagulation. The Sal B/PLGA vascular stent coating shows promising attributes, encompassing anti-inflammatory, endothelial-promoting, inhibition of smooth muscle proliferation, and anti-thrombotic properties. This array of characteristics introduces an innovative strategy with the potential to significantly enhance the efficacy of stent treatments in the future. |
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